Abstract Following induction of hemopoiesis, poly(A)–rich RNA was prepared from the heart of the tarantula, Eurypelma californicum, and translated in rabbit reticulocyte lysates. Brand manual novo nordisk princeton. In vitro translation products were immunoprecipitated with antiserum against whole dissociated Eurypelma hemocyanin. Analysis of the immunoprecipitate by sodium dodecyl sulfate/polyacrylamide gel electrophoresis revealed a set of polypeptides comigrating with authentic Eurypelma hemocyanin. The mRNA was transcribed into cDNA, clones were constructed using the pUC9 vector and probed with a synthetic 17–mer oligonucleotide probe complementary to the amino acid sequence of the ‘copper A’ binding site of chelicerate hemocyanins. One clone, pHC4, contained a 1.62–kb cDNA insert, which was subcloned into phage M13. Sequence analysis by the dideoxynucleotide chain–termination method yielded a nucleotide sequence coding for 526 amino acids of Eurypelma hemocyanin subunit e.

1. FEBS Journal
2. Voet Biochemistry Torrent

FEBS Journal

Voet Biochemistry Torrent

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